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2025-02-01 每日论文

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bioRxiv

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Rab10失活促进长时程增强过程中AMPA受体的运输和树突棘的增大 Wang, J. PDF N/A Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation
蜜月的阴暗面:通过基因组学视角重构亚洲与欧洲玫瑰育种历史 Leroy, T. PDF N/A Dark side of the honeymoon: reconstructing the Asian x European rose breeding history through the lens of genomics
在解剖追踪数据中,对纤维束进行自监督分割与表征 Sundaresan, V. PDF N/A Self-supervised segmentation and characterization of fiber bundles in anatomic tracing data
NMDA受体纳米域的跨突触分子背景 Anderson, M. C. PDF N/A Trans-synaptic molecular context of NMDA receptor nanodomains
揭示Ctla-4在肠道免疫稳态中的作用:来自新型斑马鱼炎症性肠病模型的见解 Qin, L. PDF N/A Unraveling the Role of Ctla-4 in Intestinal Immune Homeostasis: Insights from a novel Zebrafish Model of Inflammatory Bowel Disease
AI驱动的耐碱性蛋白质进化技术在大规模生产中的应用 Kang, L. PDF N/A AI-enabled Alkaline-resistant Evolution of Protein to Apply in Mass Production
JAK抑制剂停药引发短暂的促炎级联反应:重大心脏不良事件的潜在机制

在这段翻译中,"JAK Inhibitor" 被翻译为 "JAK抑制剂","Withdrawal" 翻译为 "停药","Causes" 翻译为 "引发","Transient" 翻译为 "短暂的","Proinflammatory Cascade" 翻译为 "促炎级联反应","Potential Mechanism" 翻译为 "潜在机制","Major Adverse Cardiac Events" 翻译为 "重大心脏不良事件"。整体翻译保持了原文的专业性和准确性,同时确保了中文表达的流畅性。 | Gurevic, I. | PDF | N/A | JAK Inhibitor Withdrawal Causes a Transient Proinflammatory Cascade: A Potential Mechanism for Major Adverse Cardiac Events | | RAMP3表达增加以依赖Ca2+动员的方式增强GLP-1介导的葡萄糖刺激的胰岛素分泌 | Pearce, A. | PDF | N/A | Increased RAMP3 expression enhances GLP-1 mediated Glucose Stimulated Insulin Secretion in a Manner Dependent on Ca2+ Mobilisation | | 兼性细胞内共生体Lariskella对叶足蝽(Leptoglossus zonatus)的终身适应度呈中性,并通过细胞质不亲和性在叶足蝽中传播。 | Umanzor, E. F. | PDF | N/A | The facultative intracellular symbiont Lariskella is neutral for lifetime fitness and spreads through cytoplasmic incompatibility in the leaffooted bug, Leptoglossus zonatus | | p.D372H:一种与布鲁格达综合征相关的新型SCN5A基因突变 | Xie, x. | PDF | N/A | p.D372H: A novel SCN5A mutation associated with Brugada syndrome | | 多组织转录组衰老图谱揭示了青鳉鱼中的预测性衰老生物标志物 | Costa, E. K. | PDF | N/A | Multi-tissue transcriptomic aging atlas reveals predictive aging biomarkers in the killifish | | 将这段翻译成中文如下:

基准测试用于功能预测任务的序列、表达、网络和文本模型的基因嵌入 | Zhong, J. | PDF | N/A | Benchmarking gene embeddings from sequence, expression, network, and text models for functional prediction tasks | | 从元条形码数据预测生物地球化学循环的粗粒度表示 | Belcour, A. | PDF | N/A | Predicting coarse-grained representations of biogeochemical cycles from metabarcoding data | | VIVIDHA:动态高通量分析的变异预测与可视化界面 | Barasiya, T. | PDF | N/A | VIVIDHA: Variant Prediction and Visualization Interface for Dynamic High-throughput Analysis | | PRMT1作为多发性骨髓瘤关键生存依赖性靶点的治疗潜力 | Hussain, T. | PDF | N/A | Therapeutic Potential of PRMT1 as a Critical Survival Dependency Target in Multiple Myeloma | | 对PTEN蛋白缺陷型前列腺肿瘤的转录网络分析揭示了显著的基质重编程和衰老旁分泌通讯的迹象 | Rondon-Lorefice, I. | PDF | N/A | Transcriptional network analysis of PTEN protein-deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication | | 在丙戊酸诱导的自闭症模型狨猴中,亲属关系发声动力学的改变 | Mimura, K. | PDF | N/A | Altered kinship vocal dynamics in marmosets with valproic acid-induced model of autism | | 在自然视觉过程中,动作特征主导了皮层的表征。 | Jung, H. | PDF | N/A | Action features dominate cortical representation during natural vision | | 全脑同步与fMRI运动任务数据中的预期。 | Strindberg, M. | PDF | N/A | Whole-brain entrainment and anticipation in fMRI motor task data. | | 几何因素对哺乳动物大脑区域组织的影响 | Pang, J. C. | PDF | N/A | Geometric influences on the regional organization of the mammalian brain | | 自闭症儿童早期大脑过度生长的细胞机制:GPX4水平升高与抗铁死亡能力增强 | Chen, S. | PDF | N/A | Cellular mechanisms of early brain overgrowth in autistic children: elevated levels of GPX4 and resistance to ferroptosis | | 平行层次编码在人类听觉皮层和循环自动语音识别系统中的语言表征 | Keshishian, M. | PDF | N/A | Parallel hierarchical encoding of linguistic representations in the human auditory cortex and recurrent automatic speech recognition systems | | Tsc1在小脑浦肯野神经元中的缺失会破坏轴突起始段,从而损害神经元的兴奋性和小脑功能。 | Brown, S. P. | PDF | N/A | Tsc1 Deletion in Purkinje Neurons Disrupts the Axon Initial Segment, Impairing Excitability and Cerebellar Function | | 在人类细胞和组织中绘制GDF15臂的综合应激反应图谱 | Smith, J. L. M. | PDF | N/A | Mapping the GDF15 Arm of the Integrated Stress Response in Human Cells and Tissues | | 肌强直性营养不良细胞模型中DMPK和MBNL1表达的表征:药物筛选平台 | Lopez-Martinez, A. | PDF | N/A | Characterisation of DMPK and MBNL1 expression in cell models of Myotonic Dystrophy: A platform for drug screening | | 通过多维化学蛋白质组学揭示致癌KRAS信号传导 | Kabella, N. | PDF | N/A | Illuminating oncogenic KRAS signaling by multi-dimensional chemical proteomics | | 探索PEDOT电极在血管内皮细胞电刺激中的作用 | Vitecek, J. | PDF | N/A | Exploring the Role of PEDOT Electrodes in Electrostimulation of vascular endothelial cells | | 人类多能干细胞分化过程中细胞命运潜能和钙粘蛋白转换对原始条纹坐标的依赖性 | Zhu, Y. | PDF | N/A | Dependence of cell fate potential and cadherin switching on primitive streak coordinate during differentiation of human pluripotent stem cells | | 北极植物物种显示出叶绿体DNA拷贝数水平的显著差异。 | Kamenova, S. | PDF | N/A | Arctic plant species display contrasting levels of chloroplast DNA copy numbers | | 超越接触:支持区域在区分稳定与瞬时蛋白质界面中的重要作用 | Miclot, T. | PDF | N/A | Beyond contacts: the important role of the support region to distinguish stable and transient protein interfaces | | AnchoRNA:通过保守锚定区域进行全病毒基因组比对 | Eulenfeld, T. | PDF | N/A | AnchoRNA: Full virus genome alignments through conserved anchor regions | | 使用翅膀图像进行蚊子种类识别中卷积神经网络的应用潜力与限制 | Nolte, K. | PDF | N/A | Potentials and limitations in the application of Convolutional Neural Networks for mosquito species identification using wing images | | 什么是差异表达基因? | Hoerbst, F. | PDF | N/A | What is a differentially expressed gene? | | c-Rel通过纤维连接蛋白-整合素信号通路诱导的隔离应激抵抗和上皮间质转化(EMT)激活驱动胰腺癌转移 | Bakırdögen, D. | PDF | N/A | c-Rel drives pancreatic cancer metastasis through Fibronectin-Integrin signaling-induced isolation stress resistance and EMT activation | | 组蛋白去乙酰化酶抑制剂Largazole通过抑制SP1和BRD4来使一部分超级增强子失活,并导致有丝分裂染色体错位。 | Sanchez, G. J. | PDF | N/A | Histone Deacetylase Inhibitor Largazole Deactivates A Subset of Superenchancers and Causes Mitotic Chromosome Mis-alignment by Suppressing SP1 and BRD4 | | AMPK信号通路在人类肌腱病中失调,且AMPKα1的缺失会导致小鼠跟腱的细胞、基质和机械功能异常。 | Hold, L. A. | PDF | N/A | AMPK signaling is dysregulated in human tendinopathy and loss of AMPKα1 leads to cell, matrix and mechanical dysfunction in mouse Achilles tendon | | 水杨酸通过葡萄糖-雷帕霉素靶蛋白-E2Fa途径抑制拟南芥主根生长 | Rawat, S. S. | PDF | N/A | Salicylic acid represses primary root growth through the Glucose-Target of Rapamycin-E2Fa pathway in Arabidopsis | | 一种多价泛埃博拉病毒纳米颗粒疫苗在啮齿动物中提供了对适应性扎伊尔和苏丹病毒致死性感染的保护 | Brunette, N. | PDF | N/A | A Multivalent Pan-Ebolavirus Nanoparticle Vaccine Provides Protection in Rodents from Lethal Infection by Adapted Zaire and Sudan Viruses | | 高度有害变异在人类祖先群体中的分布 | Stolyarova, A. | PDF | N/A | The distribution of highly deleterious variants across human ancestry groups | | 对唐氏综合症模型小鼠中影响性别特异性骨骼缺陷的三倍体基因进行遗传分析 | Sloan, K. | PDF | N/A | Genetic analysis of triplicated genes affecting sex-specific skeletal deficits in Down syndrome model mice | | 条件性肌营养不良蛋白缺失对肌肉发育、神经行为以及细胞外基质通路产生了深远的影响。 | Karuppasamy, M. | PDF | N/A | Conditional Dystrophin ablation causes profound effects on muscle development, neurobehavior, and extracellular matrix pathways | | Ku抑制人类细胞中RNA介导的先天免疫反应,以适应灵长类特有的Alu序列扩增。 | Zhu, Y. | PDF | N/A | Ku suppresses RNA-mediated innate immune responses in human cells to accommodate primate-specific Alu expansion. | | 《MetaProteome Investigation 2的批判性评估(CAMPI-2):多实验室评估样本处理方法以稳定粪便微生物组用于功能分析》

这篇文章介绍了一个名为CAMPI-2的研究项目,该项目通过多个实验室的合作,评估了不同的样本处理方法,旨在稳定粪便中的微生物组,以便进行更准确的功能分析。研究的目标是找到最有效的方法来保存和处理粪便样本,从而确保微生物组的稳定性和功能性在后续分析中得以保持。 | Tanca, A. | PDF | N/A | Critical Assessment of MetaProteome Investigation 2 (CAMPI-2): Multi-laboratory assessment of sample processing methods to stabilize fecal microbiome for functional analysis | | 美国国立卫生研究院(NIH)大脑计划在系统和计算神经科学领域的影响,2014-2023年 | Bader, F. | PDF | N/A | The NIH BRAIN Initiatives Impacts in Systems and Computational Neuroscience, 2014-2023 | | 通过呼吸相位触发的配对关联刺激增强运动皮层可塑性 | Baker, S. N. | PDF | N/A | Boosting Motor Cortex Plasticity through Respiratory Phase-Triggered Paired Associative Stimulation | | 实验性心房颤动中时间依赖性的线粒体重塑及其潜在治疗意义 | Nattel, S. | PDF | N/A | Time-dependent Mitochondrial Remodeling in Experimental Atrial Fibrillation and Potential Therapeutic Relevance | | 青春期晚间暴露于蓝光会诱发回避行为并重塑内侧杏仁核回路。 | Bonilla, P. | PDF | N/A | Evening blue light exposure during adolescence induces avoidance behaviors and rewires medial amygdala circuit. | | 长期吗啡戒断期间的焦虑样行为是由肠道微生物失调驱动的,并通过益生菌治疗得到缓解。 | Oppenheimer, M. | PDF | N/A | Anxiety-like behavior during protracted morphine withdrawal is driven by gut microbial dysbiosis and attenuated with probiotic treatment | | TRAF6通过Parkin依赖性和非依赖性线粒体自噬整合先天免疫信号以调节葡萄糖稳态 | Levi-DAncona, E. | PDF | N/A | TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and -independent mitophagy | | R-Spondin1通过在小鼠和人类中差异表达LGR4/5/6来调节肠神经前体细胞的命运。 | Scharr, M. | PDF | N/A | R-Spondin1 regulates fate of enteric neural progenitors via differential LGR4/5/6-expression in mice and humans | | 在炎症期间,体内痛觉外周末梢的传出信号传导增强。 | Gershon, D. | PDF | N/A | Efferent signaling along nociceptive peripheral terminals in vivo is enhanced during inflammation | | 这段文字可以翻译为:一个脑干-丘脑回路用于对冷痛的情感-动机反应。 | Reddy, P. | PDF | N/A | A brainstem-thalamic circuitry for affective-motivational responses to cold pain | | 使用邻近标记技术同时分析神经细胞类型的天然状态蛋白质组和转录组 | Ramelow, C. C. | PDF | N/A | Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labeling | | 一种用于自适应等时神经刺激的生物电路由器 | Sahai, E. | PDF | N/A | A Bioelectric Router for Adaptive Isochronous Neurostimulation | | 通过剪接因子RS2Z36调控番茄果实早期生长 | Vraggalas, S. | PDF | N/A | Regulation of early-stage tomato fruit growth by the splicing factor RS2Z36 | | 早期对无意识恐惧和中性面孔的神经反应——一项ERP研究 | Bruchmann, M. | PDF | N/A | Early neural responses to nonconscious fearful and neutral faces - an ERP study | | 建模蜂蜜支付意愿:感官特征与脑电神经指标的作用 | Zaripova, J. | PDF | N/A | Modelling the willingness to pay for honey: the role of sensory characteristics and EEG neurometrics | | FETCH技术实现了在果蝇体内对膜蛋白进行荧光标记,并具备时空控制能力。 | Rostam, K. D. | PDF | N/A | FETCH enables fluorescent labeling of membrane proteins in vivo with spatiotemporal control in Drosophila | | 肠道-大脑液压机制:大脑的运动和脑脊液循环是由与腹部的机械耦合驱动的。 | Garborg, C. S. | PDF | N/A | Gut-Brain Hydraulics: Brain motion and CSF circulation is driven by mechanical coupling with the abdomen | | 人类神经元阿尔茨海默病模型揭示衰老细胞清除疗法转化的障碍 | Walton, C. C. | PDF | N/A | A Human Neuron Alzheimer's Disease Model Reveals Barriers to Senolytic Translatability | | 脊髓中的代谢重编程推动了疼痛向慢性化的转变。 | Tagne, A. M. | PDF | N/A | Metabolic reprogramming in the spinal cord drives the transition to pain chronicity | | 一种独特的两辅料热稳定口服胰岛素片剂配方:初步结果 | Lal, M. | PDF | N/A | A unique two-excipient heat stable tablet formulation of oral insulin: preliminary results | | 阿尔茨海默病和唐氏综合症细胞模型中的多胺生物合成失调 | Paredes, D. | PDF | N/A | Polyamine biosynthesis dysregulation in Alzheimer's disease and Down syndrome cellular models | | 使用腺病毒-抗体分子胶复合物进行体内靶向基因传递 | Rice-Boucher, P. J. | PDF | N/A | In vivo targeted gene delivery using Adenovirus-antibody molecular glue conjugates | | 重新利用他汀类药物和吩噻嗪类药物治疗化疗耐药性神经母细胞瘤。 | Radke, K. | PDF | N/A | Repurposing statins and phenothiazines to treat chemoresistant neuroblastoma. | | 一种靶向NaV1.8通道的小脂肽可减轻骨关节炎疼痛行为并预防骨侵蚀 | Rodriguez, R. | PDF | N/A | A small lipidated peptide targeting NaV1.8 channels attenuates osteoarthritic pain behavior and prevents bone erosion | | 代谢模型揭示了通过工程化玉米与根瘤菌和丛枝菌根真菌共生所带来的协同生长效益。 | Kaste, J. A. M. | PDF | N/A | Metabolic modeling reveals synergistic growth benefits of engineering maize symbiosis with rhizobia and arbuscular mycorrhizal fungi | | 健康年轻人和老年人中神经分化与探索性眼球运动之间的关系 | Srokova, S. | PDF | N/A | The relationship between neural differentiation and exploratory eye movements in healthy young and older adults | | 蓝莓(BlueBerry):在自由活动的动物中进行闭环无线光遗传学操控 | Nourizonoz, A. | PDF | N/A | BlueBerry: Closed-loop wireless optogenetic manipulation in freely moving animals | | 隐式运动学习中的内部与外部错误的贝叶斯决策模型 | Kim, H. E. | PDF | N/A | A Bayesian decision-making model of implicit motor learning from internal and external errors | | Dicer在Müller胶质细胞中的缺失会导致一系列明确的病理事件,最初表现为视锥细胞功能障碍。 | Larbi, D. | PDF | N/A | Dicer loss in Muller glia leads to a defined sequence of pathological events beginning with cone dysfunction | | B细胞亚群在吞噬作用和随后将抗原呈递给同源T细胞的能力上存在差异。 | Morelli, D. M. | PDF | N/A | B cell subsets have different capacities for phagocytosis and subsequent presentation of antigen to cognate T cells | | 在Neonectria magnoliae中,两种形态上不同的专化型在木兰科宿主Liriodendron tulipifera(鹅掌楸)和Magnolia fraseri(弗雷泽木兰)上的致病性存在差异。 | Petronek, H. M. | PDF | N/A | Two morphologically distinct formae speciales in Neonectria magnoliae differ in their virulence on Magnolia family hosts Liriodendron tulipifera and Magnolia fraseri | | 在拟杆菌门中发现一种独特的BAM复合物 | Silale, A. | PDF | N/A | Discovery of a distinct BAM complex in the Bacteroidetes | | 弓形虫在活跃感染期间会先发制人,迅速抑制巨噬细胞的免疫反应。 | Murzaa, D. | PDF | N/A | Toxoplasma strikes preemptively to swiftly suppress macrophage immune response during active infection | | 生成性潜在扩散语言模型产生抗感染合成肽 | Torres, M. D. T. | PDF | N/A | Generative latent diffusion language modeling yields anti-infective synthetic peptides | | 李斯特菌(Listeria monocytogenes)通过细胞间传播绕过营养限制,使细胞内细菌得以复制。 | Radhakrishnan, P. | PDF | N/A | Listeria monocytogenes cell-to-cell spread bypasses nutrient limitation for replicating intracellular bacteria | | 酵母磷酸果糖激酶β亚基具有ATP依赖的RNA解旋活性,并调节细胞周期进程。 | Albihlal, W. | PDF | N/A | The yeast phosphofructokinase β-subunit has ATP-dependent RNA unwinding activity and modulates cell cycle progression | | TENT2介导的转录后单腺苷酸化终止了人类RNA聚合酶III转录本的3'端加工,并促进了7SL RNA的生物合成。 | Ocheltree, C. | PDF | N/A | Post-transcriptional monoadenylation by TENT2 terminates human RNA polymerase III transcript 3' end processing and promotes 7SL RNA biogenesis | | 革兰氏阴性土壤细菌对四环素暴露的早期转录后反应揭示了DUF1127基因的意外衰减机制 | Kothe, J. A. F. | PDF | N/A | Early posttranscriptional response to tetracycline exposure in a gram-negative soil bacterium reveals unexpected attenuation mechanism of a DUF1127 gene | | 基于数据库增强的Transformer大语言模型在基因-表型关系映射中实现高准确率 | Suhardi, V. | PDF | N/A | Database-Augmented Transformer-Based Large Language Models Achieve High Accuracy in Mapping Gene-Phenotype Relationships | | 通过基因组辅助育种提高美洲栗对两种入侵病原体的抗性 | Westbrook, J. W. | PDF | N/A | Improving American chestnut resistance to two invasive pathogens through genome-enabled breeding | | 蛋白酶体抑制增强溶酶体介导的靶向蛋白质降解 | Elshazly, A. M. | PDF | N/A | Proteasome Inhibition Enhances Lysosome-mediated Targeted Protein Degradation | | SCGclust: 使用图自编码器整合单核苷酸变异(SNVs)和拷贝数变异(CNAs)的单细胞图聚类方法 | Potu, T. | PDF | N/A | SCGclust: Single Cell Graph clustering using graphautoencoders integrating SNVs and CNAs | | ECD与ERBB2合作,通过上调未折叠蛋白反应和糖酵解来促进肿瘤发生。 | Kennedy, B. B. | PDF | N/A | ECD co-operates with ERBB2 to promote tumorigenesis through upregulation of unfolded protein response and glycolysis | | 基于工程化套索肽的强效和选择性双重αvβ6/8抑制剂克服免疫检查点抑制剂耐药性 | Lechner, A. | PDF | N/A | Overcoming Immune Checkpoint Inhibitor Resistance via Potent and Selective Dual αvβ6/8 Inhibitors Based on Engineered Lasso Peptides | | 食管腺癌在放化疗后复发主要由基底样亚型主导。 | Hoppe, S. | PDF | N/A | Esophageal adenocarcinoma relapse after chemoradiation is dominated by a basal-like subtype | | 变构抑制可挽救由催化失活的Shp2引起的脑积水 | Makrides, N. | PDF | N/A | Allosteric inhibition rescues hydrocephalus caused by catalytically inactive Shp2 | | 局部尿路上皮细胞驱动的逼尿肌收缩 | Alom, F. | PDF | N/A | Local urothelial cell-driven detrusor contractions | | 当替代率在不同基因座间变化时,物种树估计的样本复杂度下界 | Hill, M. | PDF | N/A | Lower Bounds on the Sample Complexity of Species Tree Estimation when Substitution Rates Vary Across Loci | | 发现和优化一种基于胍基腙的小分子以替代bFGF用于细胞培养应用 | Feofanov, M. | PDF | N/A | Discovery and optimization of a guanylhydrazone-based small molecule to replace bFGF for cell culture applications | | 研究在癌症环境中对间充质基质细胞来源的细胞外囊泡的免疫反应 | Gilligan, K. E. | PDF | N/A | Investigation of immune response to Mesenchymal Stromal Cell-derived Extracellular Vesicles in the cancer setting | | 没有外群情况下的极化SNPs | Liang, J. | PDF | N/A | Polarizing SNPs without outgroup | | 孕酮对早产和足月分娩中宫颈成熟度的调节 | Yellon, S. | PDF | N/A | Progesterone regulation of cervix ripening in preterm and term birth | | 基因模块-性状网络分析揭示了与阿尔茨海默病相关的细胞类型特异性系统和基因 | de Paiva Lopes, K. | PDF | N/A | Gene module-trait network analysis uncovers cell type specific systems and genes relevant to Alzheimers Disease | | Grin2a功能障碍通过破坏LC对mPFC回路的调节损害认知灵活性 | Hosseini, H. | PDF | N/A | Grin2a Dysfunction Impairs Cognitive Flexibility by Disrupting LC Modulation of mPFC Circuits | | VIP中间神经元通过在新环境中产生短暂的去抑制作用来控制海马位置细胞的重映射。 | Neubrandt, M. | PDF | N/A | VIP interneurons control hippocampal place cell remapping through transient disinhibition in novel environments. | | 使用rdeval对大规模测序数据进行评估 | Formenti, G. | PDF | N/A | Evaluation of sequencing reads at scale using rdeval |

medRxiv

标题 作者 PDF链接 代码仓库 Title
非侵入性检测皮层扩散性去极化与高分辨率直流电-脑电图的概念验证研究 Brown, B. R. PDF N/A Proof-of-Concept Validation of Noninvasive Detection of Cortical Spreading Depolarization with High Resolution Direct Current-Electroencephalography
低铁血症通过避免心肌反应性铁风暴,降低了ST段抬高型心肌梗死(STEMI)后主要不良心血管事件的长期风险。 Vera-Aviles, M. PDF N/A Hypoferremia reduces long-term risk of major adverse cardiovascular events after STEMI by averting the myocardial reactive iron storm
循环细胞因子水平与卒中后5年血管复发:一项多中心前瞻性队列研究 Zhang, L. PDF N/A Circulating cytokine levels and 5-year vascular recurrence after stroke: a multicenter prospective cohort study